Development of a New Non-Invasive Approach to Treat of Infantile Hemangioma with Growth-Inhibiting Endogenous Factors (Non-Invasive Approach to Treat of Infantile Hemangioma with Endogenous Factors)
Diana Dzidziguri*, Elza Markarashvili, Irina Modebadze, Levan Rusishvili, Nino Kharaishvili, Tornike Leverashvili, Zurab Vadachkoria, Giorgi Ghibradze, Lia Dzidziguri and Manana Berulava
Abstract
Background: Infant hemangioma is a benign vascular tumor, the main reason for the development of which is genetic disorders occurring in embryogenesis. It should be noted that all methods of treating the disease, both general and local, have potential negative risks and side effects. The identification of endogenous regulatory factors and researching the possibilities of using them for treatment is relevant. There is the thermostable proteins complex (TPC) which has been identified in the cells of phylogenetically distant almost all organisms, which has the ability to decrease the mitotic activity of cells by inhibition of transcription. In the experimental model of hemangioma, it has been established that inhibition of capillary growth can only be achieved by injection. The aim of the work was to develop a new, less invasive method of hemangioma treatment with endogenous factors.
Research Object and Material: Adolescent white rats and adult hens; the liver and peripheral blood of adolescent rats and the liver of the adult hens.
Methods: Isolation of TPC from hens` liver; immobilization of TPC in the polyvinyl alcohol functional polymer; determination of colchicine mitotic index; immunohistochemical analysis.
Results: The polyvinyl alcohol functional polymer does not effect on the peripheral blood leukocytes and on morphology of the adolescent rats` liver. The hen's liver TPC injected into the functional polymer retains the ability to suppress the proliferative activity of the homologous tissue of adolescent rats. At the same time, based on immunohistochemical analysis amount of ki67 positive cells is increased. Similar result was obtained on the mititic activity of the adolescent rats’ liver when the exogenous calmodulin introdiused by polimer film.
Conclusion:
1. The polyvinyl alcohol functional polymer does not have a negative effect on the peripheral blood and the liver histoarchitectonics of the adolescent white rats.
2. The protein complex immobilized into a functional polymer and its active component, calmodulin, retains the ability to suppress the proliferative activity of the adolescent rat liver.
3. There is a possibility of therapeutic use of polyvinyl alcohol functional polymer film and exogenous calmodulin immobilized into it.